微miRNA-374c-5p通过TATA-框结合蛋白相关因子7介导的DEPDC1转录调控抑制乳腺癌发展
乳腺癌是最普遍困扰女性的癌症,并且其发病率和死亡率逐年增加。异常表达的MicroRNA(miRNA)在生物学过程和各种肿瘤的进展中具有多种作用。然而,对我们而言,检查更多潜在的分子机制来治疗乳腺癌患者至关重要。本文探讨了miR-374c-5p在乳腺癌中的表达水平及其潜在的调控机制。我们的研究发现,miR-374c-5p在乳腺癌中低水平表达。 miR-374c-5p的上调抑制了细胞的增殖,迁移以及上皮-间质转化(EMT),并诱导了乳腺癌细胞的细胞凋亡。此外,我们得出结论,miR-374c-5p与TAF7相互作用并下调其表达。此外,miR-374c-5p通过介导TAF7调制了包含1的DEP域(DEPDC1)。最后,救援分析表明miR-374c-5p通过TAF7介导的DEPDC1转录调控抑制了乳腺癌的发展。我们发现,过表达的miR-374c-5p通过TAF7调控的DEPDC1转录调控抑制乳腺癌的发展,这可能是癌症诊断和治疗策略中一个新颖而重要的组成部分。**
MicroRNA-374c-5p inhibits the development of breast cancer through TATA-box binding protein associated factor 7-mediated transcriptional regulation of DEP domain containing 1
Breast cancer is the most pervasive cancer tormenting women, with increasing incidence and mortality rates year after year. MicroRNAs (miRNAs) with abnormal expression has various effects in biological processes and progression in diverse tumors. Nevertheless, it is vitally crucial for us to inspect more underlying molecular mechanisms for the therapy of patients with breast cancer. In the paper, we inquired the expression level and potential regulation mechanism of miR-374c-5p in breast cancer. Our research found out that miR-374c-5p was low-level expressed in breast cancer. Upregulation of miR-374c-5p repressed cell proliferation, migration, and also epithelial-mesenchymal transition (EMT), and induced cell apoptosis of breast cancer cells. Further, we concluded that miR-374c-5p interacted with TAF7 and downregulated its expression. Moreover, miR-374c-5p modulated DEP domain containing 1 (DEPDC1) through mediating TAF7. Finally, rescue assays represented that miR-374c-5p suppressed breast cancer development via TAF7-mediated transcriptional regulation of DEPDC1. We uncovered that overexpressed miR-374c-5p inhibited the development of breast cancer via TAF7-regulated transcriptional regulation of DEPDC1, which may be a novel and vital proportion of cancer diagnosis and treatment strategies.
pmid: 31162714 J Cell Biochem 影响因子: 3.448 发表日期: 20190604 官网 免费下载
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