通过RNA测序检测头颈癌中的可变剪接事件 - 更新
背景:选择性剪接(AS)是一种调节机制,用于从同一基因中产生多种形式的成熟信使RNA(mRNA)。 RNA测序(RNA-Seq)是一项先进的技术,已被不同的研究用于发现头颈癌(HNC)的AS机制。迄今为止,尚无可用的综述可以将这些研究的主要发现告知我们。因此,我们旨在按照PRISMA指南进行系统的文献检索,以研究通过RNA-Seq研究确定的HNC中的AS事件。 结果:总共鉴定出5条记录,这些记录利用RNA-Seq数据鉴定HNC中的AS事件。这些记录中使用了五种软件来识别AS事件。在5个记录中有4个发现受AS影响的两个基因即MLL3和RPS9是常见的。同样,在至少3个记录中鉴定出38个基因相似。 结论:HNC中的选择性剪接是基因表达的多方面调控机制。可以使用不同的生物信息学工具通过RNA-Seq进行研究。反复发现基因MLL3和RPS9与HNC相关,但是需要进一步的功能验证。**
Events of alternative splicing in head and neck cancer via RNA sequencing - an update
BACKGROUND: Alternative splicing (AS) is a regulatory mechanism used to create many forms of mature messengers RNAs (mRNAs) from the same gene. Sequencing of RNA (RNA-Seq) is an advanced technology, which has been utilized by different studies to find AS mechanisms in head and neck cancer (HNC). Hitherto, there is no available review that could inform us of the major findings from these studies. Hence, we aim to perform a systematic literature search following PRISMA guidelines to study AS events in HNC identified through RNA-Seq studies. RESULTS: A total of five records were identified that utilized RNA-Seq data for identifying AS events in HNC. Five software was used in these records to identify AS events. Two genes influenced by AS i.e. MLL3 and RPS9 were found to be common in 4 out of 5 records. Likewise, 38 genes were identified to be similar in at least 3 records. CONCLUSIONS: Alternative splicing in HNC is a multifaceted regulatory mechanism of gene expression. It can be studied via RNA-Seq using different bioinformatics tools. Genes MLL3, as well as RPS9, were repeatedly found to be associated with HNC, however needs further functional validation.
pmid: 31159745 BMC Genomics 影响因子: 3.501 发表日期: 20190603 官网 免费下载
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