右旋糖酐包裹氧化铁纳米颗粒递送miR-29a到乳腺癌细胞系
近年来,miRNA已与癌症和其他疾病的分子途径相关。 miRNA表达水平的变化在肿瘤发生中具有抑制作用。尽管如此,由于快速的酶促降解,miRNA的生物利用度差是癌症治疗中的关键障碍。在这项研究中,我们设计了基于葡聚糖的氧化铁纳米颗粒,用于将miR-29a递送至乳腺癌细胞,并分析了其体外治疗效果。结果表明,装载有miR29a的葡聚糖包被的磁性纳米颗粒的存在增强了miR-29a的选择性递送。此外,miR-29a复合纳米颗粒导致抗凋亡基因的下调。这些结果为进一步研究miR-29a复合磁性纳米粒子在乳腺癌治疗中的可能用途铺平了道路。**
Dextran coated iron oxide nanoparticle for delivery of miR-29a to breast cancer cell line
In the last years, miRNAs have been associated with molecular pathways of cancer and other diseases. The change of expression level of miRNA has an inhibitory role in tumorigenesis. Nevertheless, the poor bioavailability of miRNA due to the rapid enzymatic degradation is a critical handicap in cancer therapy. In this study, we designed dextran-coated iron oxide-based nanoparticle for the delivery of miR-29a to breast cancer cells and analyzed its therapeutic efficacy in vitro. Results indicated that the presence of dextran-coated magnetic nanoparticles, loaded with miR29a, enhanced the selective delivery of miR-29a. Further, miR-29a complex nanoparticles caused down-regulation of anti-apoptotic genes. These results pave the way for further investigations into the possible use of miR-29a complex magnetic nanoparticles for breast cancer therapy.
pmid: 31159615 Pharm Dev Technol 影响因子: 2.347 发表日期: 20190604 官网 免费下载
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