宫颈癌前细胞增殖的生物学特征
高度鳞状上皮内病变(HSIL)被认为是可以发展为宫颈癌的癌前病变。然而,仅凭形态就很难有效地将这些癌前细胞与癌细胞区别开来。另外,癌前细胞和癌细胞之间在生物学行为方面的差异仍然不清楚。我们以前曾从人类正常子宫颈组织培养原代正常子宫宫颈角质形成细胞,并从小型肿瘤性宫颈组织中自然感染了人类乳头瘤病毒的子宫颈癌前细胞。在这里,我们扩大了研究范围,以进一步观察宫颈癌前细胞在细胞和分子水平上的体外增殖特性。在这项研究中,我们发现癌前细胞的生长速度明显快于正常宫颈细胞,而慢于Caski细胞。然而,在分子水平上,这种癌前细胞的增殖能力与子宫颈癌细胞的增殖能力相似。这些结果表明,细胞的周围环境可能在宫颈癌的发展中起重要作用,这为进一步研究子宫颈癌前病变和癌性病变提供了重要依据。**
Biological Characteristics of Cervical Precancerous Cell Proliferation
High-grade squamous intraepithelial lesions (HSILs) are regarded as precancerous lesions that can progress to cervical carcinoma; however, it is very difficult to effectively differentiate these precancerous cells from cancerous cells based on morphology alone. Additionally, the difference between precancerous cells and cancerous cells in regard to biological behaviour remains unclear. We previously cultured primary normal uterine cervical keratinocytes from human normal cervical tissue and cervical precancerous cells that were naturally infected with human papillomavirus from small-sized neoplastic cervical tissues. Here, we extended our study to further observe the in vitro proliferative characteristics of cervical precancerous cells at the cellular and molecular levels. In this study, we found that the growth rate of precancerous cells was significantly faster than that of normal cervical cells and slower than that of Caski cells. However, the proliferative capacity of such precancerous cells was similar to that of cancerous cells of the cervix at the molecular level. These results suggest that the surrounding environment of the cells may play an important role in the development of cervical cancer, which provides an important basis for the further study of precancerous and cancerous lesions of the cervix.
pmid: 31157301 Open Med (Wars) 影响因子: 0.0 发表日期: 20190101 官网 免费下载
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