通过单细胞测序广泛探索癌症T细胞异质性
人T细胞是高度异质的群体,可以通过其T细胞受体(TCR)识别多种抗原。肿瘤细胞显示出大量的抗原,这些抗原可作为潜在的识别靶标,从而使肿瘤微环境中的T细胞更加复杂。将TCR与单个T细胞的转录信息之间建立联系对于研究病理条件下T细胞群体内的克隆扩增将非常有趣。单细胞RNA测序(scRNA-seq)的进步已允许对T细胞进行全面分析。在这篇综述中,我们简要描述了使用单细胞RNA测序对肿瘤微环境T细胞的研究进展,然后讨论了如何使用scRNA-seq解决健康和疾病中免疫系统的异质性。最后,我们指出了该领域的未来方向以及免疫疗法的潜力。**
Extensive exploration of T cell heterogeneity in cancers by single cell sequencing
Human T cells are a highly heterogeneous population and can recognize a wide variety of antigens by their T cell receptors (TCRs). Tumor cells display a large repertoire of antigens that serve as potential targets for recognition, thus making T cells in the tumor micro-environment more complicated. Making a connection between TCRs and the transcriptional information of individual T cells will be interesting for investigating clonal expansion within T cell populations under pathologic conditions. Advances in single cell RNA-sequencing (scRNA-seq) have allowed for comprehensive analysis of T cells. In this review, we briefly describe the research progress on tumor micro-environment T cells using single cell RNA sequencing, and then discuss how scRNA-seq can be used to resolve immune system heterogeneity in health and disease. Finally, we point out future directions in this field and potential for immunotherapy.
pmid: 31156311 Chin J Cancer Res 影响因子: 0.0 发表日期: 20190401 官网 免费下载
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